Identification of Antibiotic-Resistant Genes and Effect of Garlic Ethanolic Extract on Mycobacterium tuberculosis Isolated from Patients in Zabol, Iran

Background: Mycobacterium is a genus of Actinobacteriaceae and the family, including important pathogens, such as tuberculosis (i.e., cause tuberculosis) leprae leprosy). Tuberculosis still major death in human societies. Objectives: The current study aimed to evaluate effect ethanolic extract garlic on isolated from patients Zabol, Iran, investigate presence antibiotic-resistant genes tuberculosis. Methods: Garlic (Allium sativum) was collected leaf obtained. In this study, 50 strains were obtained Zabol. minimum inhibitory concentration (MIC) bactericidal (MBC) determined. Some antibiotics, isoniazid, pyrazinamide, ethambutol, amikacin, streptomycin, rifampicin, used for positive control. Genomic deoxyribonucleic acid extracted by sodium dodecyl sulfate method. Furthermore, genes, namely KatG, PncA, embC, embA1, embA2, embB1, embB2, rrs, rpsL, ropB, investigated using polymerase chain reaction. Results: lowest MIC MBC against 3.25 7.5 ppm, respectively. highest 60 120 Following investigation tuberculosis, it determined that contains embB1 genes. resistance rifampin (81%) then amikacin (76.6%) belonging ropB rrs Conclusions: results present showed very effective most mycobacteria rrs. Although not recommended directly use study. Therefore, required perform clinical trials confirm results.